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Histological findings also support our results. A well-known antioxidant vitamin C was included in this study to compare the antioxidant potency of AEPD. Combining all, results suggest that AEPD protects murine liver against CCl 4 -induced oxidative impairments probably via its antioxidative property. Hepatoprotective effects of the polysaccharide isolated from Tarphochlamys affinis Acanthaceae against CCl 4 -induced hepatic injury.

This study was designed to investigate the protective effects of the polysaccharide isolated from Tarphochlamys affinis PTA against CCl 4 -induced hepatotoxicity in rats. Liver injury was induced in rats by the administration of CCl 4 twice a week for 2 weeks. During the experiment, the model group received CCl 4 only; the treatment groups received various drugs plus CCl 4 , whereas the normal control group received an equal volume of saline.

Moreover, the content of hepatic malondialdehyde MDA was reduced. Histological findings also confirmed the anti-hepatotoxic characterisation. In brief, our results show that the protective effect of PTA against CCl 4 -induced hepatic injury may rely on its ability to reduce oxidative stress and suppress inflammatory responses. Protective effects of Ziyang tea polysaccharides on CCl 4 -induced oxidative liver damage in mice. This study was designed to investigate the hepatoprotective effects of the tea polysaccharides ZTPs extracted from a selenium-enriched Ziyang green tea Camellia sinensis.

ZTPs were identified as the heteropolysaccharides with glucose Administration of ZTPs in mice prior to CCl 4 significantly prevented the CCl 4 -induced increases in serum alanine aminotransferase, aspartate aminotransferase and lactic dehydrogenase, as well as hepatic malondialdehyde level.

Mice treated with ZTPs showed normal glutathione peroxidase and superoxide dismutase activities, relative to CCl 4 -treated group. ZTPs also prevented the CCl 4 -caused liver histological alteration, as indicated by histopathological evaluation. These findings demonstrate that ZTPs have protective effects against acute CCl 4 -induced oxidative liver damage. Betanin attenuates carbon tetrachloride CCl 4 -induced liver injury in common carp Cyprinus carpio L. This study investigates the protective effect of betanin against liver injury induced by carbon tetrachloride CCl 4 in common carp Cyprinus carpio L.

Moreover, the inhibition of CYP2E1 activity and oxidative stress may have significant roles in the protective effect of betanin. Prevention of CCl 4 -induced liver damage by ginger, garlic and vitamin E. The hepatoprotective effects of garlic Allium sativum , ginger Zingiber officinale and vitamin E pre-treatment against carbon tetrachloride CCl 4 -induced liver damage in male wistar albino rats were investigated.

Carbon tetrachloride 0. Serum alanine amino transferase, aspartate amino transferase and alkaline phosphatase levels, 24 h after CCl 4 administration, decreased significantly p CCl 4 -treated rats only. Lipid peroxidation expressed by serum malondialdehyde MDA concentration was assayed to assess the extent of liver damage by CCl 4 ; including the extent of hepatoprotection by garlic, ginger and vitamin E.

MDA concentration was significantly decreased p CCl 4 -alone. Histological examination of the liver revealed severe infiltration of inflammatory cells in rats treated with CCl 4 alone. However, the observed alteration in the normal architecture of the hepatic cells decreased remarkably in pre-treated rats. The electron energy was keV, and the pump current pulse duration and amplitude were 5 ns and 5 A, respectively. Electron beam pumping in a cell 4 cm long with a cavity having an output mirror transmittance of 2.

Silymarin Si is a herbal product with hepatoprotective potential, well-known for its antioxidant, anti-inflammatory, and immunomodulatory properties. We have recently demonstrated that the usual therapeutic doses of Si are capable of inhibiting the progression of incipient liver fibrosis.

We aimed at further investigating the benefits of Si administration upon liver alterations after the hepatotoxin discontinuation, using CCl 4 to induce liver injuries on rats. CCl 4 administration induces first of all oxidative stress, but other mechanisms, such as inflammation and liver fibrosis are also triggered.

The control group received sunflower oil twice a week for 8 weeks. Carboxymethyl cellulose group received sunflower oil twice a week, for 8 weeks and CMC daily, for the next 2 weeks. CCl 4 group received CCl 4 in sunflower oil, by gavage, twice a week, for 8 weeks. Silymarin for the next 2 weeks.

Our data showed that Silymarin administered after the toxic liver injury is capable of reducing inflammation and liver fibrosis. The benefits were more important for the higher dose than for the usual therapeutic dose. Exposure to the halogenated hydrocarbon carbon tetrachloride CCl 4 leads to hepatic lipid peroxidation, inflammation and fibrosis.

We showed that soluble epoxide inhibitors reduced CCl 4 -induced liver fibrosis. Three major results were obtained. Exposure to the halogenated hydrocarbon carbon tetrachloride CCl 4 leads to hepatic lipid peroxidation, inflammation, and fibrosis. In this study, we showed that soluble epoxide inhibitors reduced CCl 4 -induced liver fibrosis. To observe the protective effect of Tanreqing injection TRQ on carbon tetrachloride-induced acute hepatic injury in rats.

At 6 h after the first CCl 4 injection, the rats were divided into six groups: the model group, the control group, the diammonium glycyrrhizinate-treated group, and TRQ high, middle and low dose groups. They were injected through caudal veins, while a normal control group was set up. Their weight and liver-body ratio were observed. Hepatic inflammation was observed with HE staining. Bil, D. According to the single injection model, serum AST and T.

Bil of model rats were obviously increased at 6 h after single subcutaneous injection of CCl 4 , with disordered lobular structure in liver tissues, notable swollen liver cells and remarkable liver injury. According to the results of the multiple injection pharmacological experiment, compared with the normal group, the model group had higher serum ALT, AST, and gamma-GT activities P Rac2 deficiency attenuates CCl 4 -induced liver injury through suppressing inflammation and oxidative stress.

Oxidative stress is a leading cause to liver injury. Rac2 is a Ras-associated guanosine triphosphatase, an important molecule modulating a large number of cells and involved in the regulation of reactive oxygen species ROS. For the study described here, we supposed that Rac2 knockout protects mice against CCl 4 -induced acute liver injury. We found that Rac2 expressed highly in CCl 4 -induced liver tissues. In addition, CCl 4 -induced high expression of pro-inflammatory cytokines and chemokine were reversed by Rac2 deficiency compared to CCl 4 -treated wild type WT mice.

The data further confirmed the role of Rac2 deficiency suppressed pro-inflammatory cytokines and chemokine, as well as fibrosis-related signals. Our results indicated that Rac2 played an essential role in acute liver injury induced by CCl 4 , providing the compelling information of the effects of Rac2 on liver injury, and revealing a novel regulatory mechanism for acute liver injury. Published by Elsevier. Heat tolerance of CCl 4 -treated animals and its modification by some agents.

Significant protection against the reduction in heat tolerance by CCl 4 was provided by glucose and garlic but not by vitamin E. The reduction in heat tolerance by CCl 4 was attributed to the hypoglycemia caused by it, followed by breakdown of the thermoregulatory centres in the hypothalamus. The protective effect of glucose was attributed to the restoration of blood glucose levels and that of the garlic oil to its protective effect on hepatocytes against CCl 4 toxicity.

A single acute hepatotoxic dose of CCl 4 causes oxidative stress in the rat brain. Carbon tetrachloride CCl 4 , a hepatotoxic agent is widely used to study the toxic mechanisms in experimental animals. Increased lipid peroxidation LPO , protein carbonyls PC content and glutathione GSH depletion were observed in the brain regions of rats treated with CCl 4 which was higher than that of liver.

A drastic reduction in the activity of glutathione- S -transferase GST was seen in the brain regions which was higher than that of liver. Higher induction of oxidative stress in the brain compared to that of liver implies vulnerability of the brain for CCl 4 neurotoxicity. Our study shows that a single hepatotoxic dose of CCl 4 is equally neurotoxic to rats. We investigated the effect of grape seed proanthocyanidins GSPs on carbon tetrachloride CCl 4 -induced acute liver injury.

Hepatoprotective effect of manganese chloride against CCl 4 -induced liver injury in rats. The aim of the present study is to evaluate the protective effect of manganese chloride against carbon tetrachloride CCl 4 -induced liver injury in rats. Manganese chloride 0. Histopathological changes in the liver of different groups were also studied. Treatment with manganese chloride significantly attenuated these changes to nearly normal levels. The animals treated with manganese chloride have shown decreased necrotic zones and hepatocellular degeneration when compared to the liver exposed to CCl 4 intoxication alone.

Thus, the histopathological studies also supported the protective effect of manganese chloride. Therefore, the results of this study suggest that manganese chloride exerts hepatoprotection via promoting antioxidative properties against CCl 4 -induced oxidative liver damage. Hepatoprotective effects of Vaccinium arctostaphylos against CCl 4 -induced acute liver injury in rats.

This study was carried out to evaluate the antioxidant and hepatoprotective effects of Vaccinium arctostaphylos V. Total phenolic and total flavonoid contents as well as antioxidant activity of V. Extracts of V. Serum biochemical markers of liver injury, oxidative markers, as well as hydroxyproline HP content and histopathology of liver were evaluated. The obtained results showed that V.

Treatment of rats with V. The level of hepatic HP content was also reduced by the administration of V. Histological examination of the liver section revealed that V. These findings suggested that V. Protective effects of L-carnosine on CCl 4 -induced hepatic injury in rats. The present study was undertaken to investigate the possible protective effect of L-carnosine CAR , an endogenous dipeptide of alanine and histidine, on carbon tetrachloride CCl 4 -induced hepatic injury.

Liver injury was induced in male Sprague-Dawley rats by intraperitoneal i. At the end of six weeks, blood and liver tissue specimens were collected. Results show that CAR treatment attenuated the hepatic morphological changes, necroinflammation and fibrosis induced by CCl 4 , as indicated by hepatic histopathology scoring.

In addition, CAR treatment significantly reduced the CCl 4 -induced elevation of liver-injury parameters in serum. CAR treatment also combatted oxidative stress; possibly by restoring hepatic nuclear factor erythroid 2-related factor 2 Nrf-2 levels.

The results suggest that these beneficial, protective effects are due, at least in part, to its anti-oxidant, anti-inflammatory and anti-fibrotic activities. Protective effect of chitosan from Sepia kobiensis Hoyle cuttlebone against CCl 4 induced hepatic injury. Carbon tetrachloride CCl 4 is a potent hepatotoxic agent causing hepatic necrosis and it is widely used in animal models for induction of acute and chronic liver damage.

Rats treated with chitosan against CCl 4 toxicity showed significantly decreased levels of ALT and AST activities, total cholesterol, triglyceride and free fatty acid in plasma and tissue. Whereas the treatment with chitosan along with CCl 4 showed markedly increased level of hepatic and circulatory in SOD, CAT, GPx and reduced glutathione and decreased the malondialdehyde level.

Histopathological observations proved the marked hepatoprotective effect of chitosan. The CCl 4 induced alterations in circulatory and hepatic antioxidant defense system were normalized by chitosan and it could be concluded that the hepatoprotective effect of chitosan may be due to its antioxidant and antilipidemic properties. Several studies have shown the hepatoprotective effect of the consumption of coffee and tea, which is mainly attributed to caffeine.

Many experimental studies have demonstrated this effect; however, these studies used high caffeine doses that are not related to human consumption. The aim of this study was to evaluate the hepatoprotective effect of low doses of caffeine on carbon tetrachloride CCl 4 -treated rats.

Liver function tests biochemical markers and functional sleeping time and histological hematoxylin-eosin and Masson trichrome stains parameters were carried out at the end of damage treatment. Moreover, CAFF prevented CCl 4 -induced prolongation in pentobarbital sleeping time and a decrease of liver fibrosis and cell death.

Our results demonstrated that low doses of CAFF exert a hepatoprotective effect against CCl 4 -induced liver damage in rats. Rating of CCl 4 -induced rat liver fibrosis by blood serum glycomics. Non-invasive staging of human liver fibrosis is a desirable objective that remains under extensive evaluation. Animal model systems are often used for studying human liver disease and screening antifibrotic compounds.

The aim of the present study was to investigate the potential use of serum N-glycan profiles to evaluate liver fibrosis in a rat model. Liver fibrosis and cirrhosis were induced in rats by oral administration of CCl 4. Liver injury was assessed biochemically alanine aminotransferase [ALT] activity, aspartate aminotransferase [AST] activity and total bilirubin and histologically.

The N-glycan profile GlycoTest was performed using DNA sequencer-assisted-fluorophore-assisted carbohydrate electrophoresis technology. In parallel, the effect of cotreatment with antifibrotic interferon-gamma IFN-gamma was studied. GlycoTest showed three glycans were significantly altered in the CCl 4 -goup. Peak 3 started at week 6, at an early stage in fibrosis development F CCl 4 -IFN-gamma group were intermediate between the CCl 4 - and the control groups.

The test can also be used as a non-invasive marker for screening and monitoring the antifibrotic activity of potential therapeutic compounds. MIPAS thermal limb emission measurements were used to derive vertically resolved profiles of carbon tetrachloride CCl 4. Consideration of peroxyacetyl nitrate PAN as an interfering species, which is jointly retrieved, and CO2 line mixing is crucial for reliable retrievals. Parts of the CO2 Q-branch region that overlap with the CCl 4 signature were omitted, since large residuals were still found even though line mixing was considered in the forward model.

However, the omitted spectral region could be narrowed noticeably when line mixing was accounted for. A new CCl 4 spectroscopic data set leads to slightly smaller CCl 4 volume mixing ratios. In general, latitude-altitude cross sections show the expected CCl 4 features with highest values of around 90 pptv at altitudes at and below the tropical tropopause and values decreasing with altitude and latitude due to stratospheric decomposition.

Other patterns, such as subsidence in the polar vortex during winter and early spring, are also visible in the distributions. The decline in CCl 4 abundance during the MIPAS Envisat measurement period July to April is clearly reflected in the altitude-latitude cross section of trends estimated from the entire retrieved data set. Protective effect of human serum amyloid P on CCl 4 -induced acute liver injury in mice.

Our data indicated that hSAP attenuated hepatic histopathological abnormalities and significantly decreased inflammatory cell infiltration and pro-inflammatory factor expression. Collectively, these findings suggest that hSAP exerts a protective effect againts CCl 4 -induced hepatic injury by suppressing the inflammatory response and hepatocyte apoptosis, potentially by inhibiting HSC activation.

Procyanidin B2 has demonstrated several health benefits and medical properties. However, its protective effects against CCl 4 -induced hepatotoxicity have not been clarified. The present study aimed to investigate the hepatoprotective effects of procyanidin B2 in CCl 4 -treated mice. Our data showed that procyanidin B2 significantly decreased the CCl 4 -induced elevation of serum alanine aminotransferase activities, as well as improved hepatic histopathological abnormalities. Moreover, CCl 4 -induced apoptosis in mouse liver was measured by terminal-deoxynucleotidyl transferase mediated nick end labeling TUNEL assay and the cleaved caspase Meanwhile, the expression of apoptosis-related proteins Bax and Bcl-xL was analyzed by Western blot.

Results showed that procyanidin B2 significantly inhibited CCl 4 -induced hepatocyte apoptosis, markedly suppressed the upregulation of Bax expression and restored the downregulation of Bcl-xL expression. Overall, the findings indicated that procyanidin B2 exhibited a protective effect on CCl 4 -induced hepatic injury by elevating the antioxidative defense potential and consequently suppressing the inflammatory response and apoptosis of liver tissues.

Effects of platelet-rich plasma on liver regeneration in CCl 4 -induced hepatotoxicity model. Numerous bioactive growth factors and cytokines in platelet-rich plasma PRP have recently made it an attractive biomaterial for therapeutic purposes. These growth factors have the potential to regenerate the injured tissues. The aim of this study was to investigate the therapeutic effects of PRP in hepatotoxic animal model.

To confirm the hepatotoxicity, 24 h after the last CCl 4 administration, blood samples were collected via cardiac puncture to assess the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein, and total bilirubin.

Twenty-four hours after blood collection, the experimental animals received a single injection of PRP 1 mL via the anterior mesenteric vein. One week later, all biochemical tests were performed again, and the rats were scarified and their livers were removed, prepared histologically, and stained. The stereological analyses were performed to evaluate the effects of PRP on histopathological features of CCl 4 -treated livers. Our findings demonstrated that application of PRP had beneficial effects on CCl 4 -induced fibrosis; however, it had detrimental effects on the total number of hepatocytes and the volume of hepatocytes and sinusoidal spaces.

Liver fibrosis, which is the pathophysiologic process of the liver due to sustained wound healing in response to chronic liver injury, will eventually progress to cirrhosis. Puerarin, a bioactive isoflavone glucoside derived from the traditional Chinese medicine pueraria, has been reported to have many anti-inflammatory and anti-fibrosis properties. However, the detailed mechanisms are not well studied yet. This study aimed to investigate the effects of puerarin on liver function and fibrosis process in mice induced by CCl 4.

As indicated by the ameliorative serum hepatic enzymes and the reduced histopathologic abnormalities, the data collected showed that puerarin can protect against CCl 4 -induced chronic liver injury. Inhibitory effect of gallic acid on CCl 4 -mediated liver fibrosis in mice. The aim of this study was to investigate the effect of gallic acid GA on liver fibrosis induced by carbon tetrachloride CCl 4.

GA was intra-gastric given for mice once a day after 2 weeks of CCl 4 induction. Animals were killed at the eighth week. Degrees of fibrosis and collagen percentage were measured. Western blotting was carried out to evaluate the changes of MMP-2 protein. HE and VG stainings showed GA in a dose-dependent manner improved significantly the fibrosis condition in CCl 4 -injured mice P CCl 4 in mice, which might be through the inhibition of hepatic stellate cell activity.

Human serum amyloid P hSAP , a member of the pentraxin family, inhibits the activation of fibrocytes in culture and inhibits experimental renal, lung, skin and cardiac fibrosis. As hepatic inflammation is one of the causes of liver fibrosis, in the present study, we investigated the hepatoprotective effects of hSAP against carbon tetrachloride CCl 4 -induced liver injury.

Moreover, CCl 4 -induced apoptosis in the mouse liver was inhibited by hSAP, as measured by terminal-deoxynucleotidyl transferase mediated nick-end labeling TUNEL assay and cleaved caspase-3 expression. Prevention of CCl 4 induced hypogonadism with Raphanus sativus seeds in rat.

Raphanus sativus seeds are used as condiment and to treat hypogonadism, various ailments of liver and kidneys. The aim of this study was to evaluate the potential protective effects of methanol extract of R. Thirty six rats were divided in to six groups with six animals in each. All treatments were given at alternate days for 15 days. Injuries in seminiferous tubules were determined in histopathology of testes.

Administration of RSME, dose dependently, markedly ameliorated the oxidative stress of CCl 4 thereby restoring the level of antioxidant enzymes, lipid peroxides, reduced glutathione, male hormones and alterations in histopathology. Chemokines are signaling molecules playing an important role in immune regulations. They are also thought to regulate brain development, neurogenesis and neuroendocrine functions.

While chemokine upsurge has been associated with conditions characterized with cognitive impairments, their ability to modulate synaptic plasticity remains ill-defined. This effect was ascribed to post-synaptic regulations, as CCL 3 did not impact paired-pulse facilitation. In accordance with these electrophysiological findings, we demonstrated that the icv injection of CCL 3 in mouse significantly impaired spatial memory abilities and long-term memory measured using the two-step Y-maze and passive avoidance tasks.

These effects of CCL 3 on memory were inhibited by Maraviroc. Altogether, these data suggest that the chemokine CCL 3 is an hippocampal neuromodulator able to regulate synaptic plasticity mechanisms involved in learning and memory functions. CCl 4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis. Sonchus arvesis is traditionally reported in various human ailments including hepatotoxicity in Pakistan. Presently we designed to assess the protective effects of methanolic extract of Sonchus arvesis against carbon tetrachloride induced genotoxicity and DNA oxidative damages in hepatic tissues of experimental rats.

Rats of normal control group were given free access of food and water add labitum. Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages. Results of the present study showed that treatment of SME reversed the activities of serum marker enzymes and cholesterol profile as depleted with CCl 4 treatment. These results reveal that treatment of SME may be useful in the prevention of hepatic stress. Atmospheric emissions of carbon tetrachloride CCl 4 are regulated by the Montreal Protocol due to its role as a strong ozone-depleting substance.

The molecule has been the subject of recent increased interest as a consequence of the so-called mystery of CCl 4 , the discrepancy between atmospheric observations and reported production and consumption. Surface measurements of CCl 4 atmospheric concentrations have declined at a rate almost 3 times lower than its lifetime-limited rate, suggesting persistent atmospheric emissions despite the ban.

In this paper, we study CCl 4 vertical and zonal distributions in the upper troposphere and lower stratosphere including the photolytic loss region, hPa , its trend, and its stratospheric lifetime using measurements from the Michelson Interferometer for Passive Atmospheric Sounding MIPAS , which operated onboard the ENVISAT satellite from to The CCl 4 zonal means show features typical of long-lived species of anthropogenic origin that are destroyed primarily in the stratosphere, with larger quantities in the troposphere and a monotonic decrease with increasing altitude in the stratosphere.

MIPAS CCl 4 measurements have been compared with independent measurements from other satellite and balloon-borne remote sounders, showing a good agreement between the different datasets. CCl 4 trends are calculated as a function of both latitude and altitude.

At the lowest altitudes sounded by MIPAS, we find trends consistent with those determined on the basis of. In the present study, we examined the role of indoleamine 2,3-dioxygenase IDO in the development of CCl 4 -induced hepatic fibrosis. In IDO-KO mice treated with CCl 4 , the number of several inflammatory cells and the expression of pro-inflammatory cytokines increased in the liver. Moreover, the treatment with l-tryptophan aggravated the CCl 4 -induced hepatic fibrosis in WT mice.

Our findings demonstrated that the IDO deficiency enhanced the inflammation in the liver and aggravated liver fibrosis in repetitive CCl 4 -treated mice. Mycobacterium tuberculosis M. Yin-Yang-1 YY1 plays a major role in the maintenance and progression of some pulmonary diseases, including pulmonary fibrosis.

However, the role of YY1 in TB remains unknown. A retrospective comparison of patients with TB and control subjects was used to measure the expression of YY1 and CCL 4 using tissue microarrays. Therefore, treatments that decrease YY1 expression may be a new therapeutic strategy against TB.

Protective effect of wedelolactone against CCl 4 -induced acute liver injury in mice. Eclipta, a traditional Chinese medicine, has been used to treat liver disease for centuries. However, the chemical basis and biological mechanisms of Eclipta remain elusive. Our data showed that WEL markedly decreased the CCl 4 -induced elevation of serum alanine aminotransferase ALT and aspartate aminotransferase AST activities, and improved hepatic histopathology changes. N-terminal kinase JNK. Moreover, results showed that WEL significantly inhibited CCl 4 -induced hepatocytes apoptosis, markedly suppressed the down-regulation of Bax and active Caspase-3 expression and accelerated the expression of Bcl Overall, the findings indicate that WEL exhibits a protective effect against CCl 4 -induced ALI in mice by enhancing the antioxidative defense system, suppressing the inflammatory response and cell apoptosis of liver.

Liver metabolomics study reveals protective function of Phyllanthus urinaria against CCl 4 -induced liver injury. Phyllanthus Urinaria L. PUL is a traditional Chinese medicine used to treat hepatic and renal disorders.

However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. Significant changes in liver metabolic profiling were observed in PUL-treated group compared with liver injury model group.

The results suggested that the potential hepatoprotective effects of PUL in attenuating CCl 4 -induced hepatotoxicity could be partially attributed to regulating L-carnitine, taurocholic acid, and amino acids metabolism, which may become promising targets for treatment of liver toxicity.

In conclusion, this study provides new insights into the mechanism of the hepatoprotection of Phyllanthus Urinaria. Published by Elsevier B. Protective effect of Anoectochilus roxburghii polysaccharide against CCl 4 -induced oxidative liver damage in mice.

This study investigated the isolation and characterization of Anoectochilus roxburghii polysaccharides ARP , and further evaluated whether ARP possessed hepatoprotective activities against CCl 4 -induced oxidative liver damage in mice.

ARP is comprised of glucose and galactose in a 1. ARP displayed significant scavenging effects against hydroxyl radical, superoxide anion radical, DPPH radical and a strong reducing power. Furthermore, hepatic histopathological changes induced by CCl 4 were significantly normalized by ARP pretreatment. These findings demonstrated that ARP possessed hepatoprotective effect against acute CCl 4 -induced liver damage by reducing lipid oxidation.

Antioxidant and protective effect of inulin and catechin grafted inulin against CCl 4 -induced liver injury. In this study, the antioxidant activity and hepatoprotective effect of inulin and catechin grafted inulin catechin-g-inulin against carbon tetrachloride CCl 4 -induced acute liver injury were investigated. Results showed that both inulin and catechin-g-inulin had moderate scavenging activity on superoxide radical, hydroxyl radical and H2O2, as well as lipid peroxidation inhibition effect.

Administration of inulin and catechin-g-inulin could significantly reduce the elevated levels of serum aspartate transaminase, alanine transaminase and alkaline phosphatase as compared to CCl 4 treatment group. Moreover, inulin and catechin-g-inulin significantly increased the levels of hepatic superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione and total antioxidant capacity, whereas markedly decreased the malondialdehyde level when compared with CCl 4 treatment group.

Notably, catechin-g-inulin showed higher hepatoprotective effect than inulin. In addition, the hepatoprotective effect of catechin-g-inulin was comparable to positive standard of silymarin. Our results suggested that catechin-g-inulin had potent antioxidant activity and potential protective effect against CCl 4 -induced acute liver injury. To study the protective effects of polysaccharides from Dendrobium huoshanense DHP against CCl 4 -induced liver injury in mice. Eighty male Kunming mice were randomly divided into normal control group, model control group, dextran control group, starch control group, hydrolyzate control group, three different dose of DPH groups consisting of high-dosage group, middle-dosage group and low-dosage group , , 50 mg x kg Each group contained ten mice.

Immunohistochemistry was used to detect the expression of TNF-alpha in hepatic tissue. Hepatic histopathological examination was observed. Liver tissue damage induced by CCl 4 was ameliorated in mice with DHP administration through histopathology examination.

Furthermore, the expression of TNF-alpha was greatly decreased in groups treated with polysaccharides. DHP has a significantly hepatoprotective effect on CCl 4 -induced acute liver injury in mice. Protective effect of DHP on the liver may be related to its function of scavenging free radicals and inhibiting lipid peroxidation and TNF-alpha expression.

Apitherapy products enhance the recovery of CCL 4 -induced hepatic damages in rats. Our objective was to identify the antioxidant properties of honeybee products from Turkey, chestnut honey, pollen, propolis, and royal jelly, and their hepatoprotective activity against CCl 4 -induced hepatic damage in rats.

Animals were fed with honeybee products for 7 days following CCl 4 injection. Development of liver damage and oxidative stress were monitored by measuring the activities of the enzymes alanine transaminase, aspartate transaminase, malondialdehyde, superoxide dismutase, and catalase. The antioxidant activities of the honeybee products were highest in propolis, followed, in order, by pollen, honey, and royal jelly.

Despite their different levels of antioxidant capacity, their roles in the prevention of liver damage induced by CCl 4 were very similar, which can be explained through their bioavailability to the treated animals. Our results suggest that honey, propolis, pollen, and royal jelly significantly enhanced the healing of CCl 4 -induced liver damage, partially due to their antioxidant properties and bioavailability.

Ponderal changes and liver marker enzymes were estimated. Hepatic collagen and histopathological studies were carried out. Hence, it can be used as a promising hepatoprotective agent. To characterize [methyl- 11 C]choline [ 11 C]choline as an oncologic PET radiopharmaceutical, [ 11 C]choline uptake in regenerating livers after partial hepatectomy as a model of typical proliferating tissue and after CCl 4 insult as that of proliferating tissue with inflammation, was studied in rats.

On the other hand, the uptake was not accelerated by CCl 4 -administration, though it significantly increased [ 14 C]TdR uptake. There were no differences of [ 11 C]choline uptake acceleration following partial hepatectomy among the three parts of the regenerating liver. The magnitude of the increase in [ 18 F]FDG uptake in the regenerating liver induced by partial hepatectomy was greater than that for [ 11 C]choline.

This might be expected given that the differentiation between proliferating tissues such as tumor and inflammatory tissue was possible by [ 11 C]choline-PET. Neutrophils alleviate fibrosis in the CCl 4 -induced mouse chronic liver injury model. Loss of Trib1 was previously shown to increase the neutrophil population in the spleen but lead to M2-like macrophage reduction.

Because M2 macrophages are anti-inflammatory and promote tissue repair by producing fibrogenic factors, we investigated liver fibrosis in Trib1 -deficient mice. Interestingly, loss of Trib1 suppressed fibrosis in the CCl 4 -induced chronic liver injury model. Trib1 knockout increased neutrophils but had a minimal effect on the macrophage population in the liver. These results suggest that neutrophils are responsible for the suppression of fibrosis in Trib1 -deficient liver.

Consistently, transplantation of Trib1 -deficient bone marrow cells into wild-type mice alleviated CCl 4 -induced fibrosis. Furthermore, expression of chemokine C-X-C motif ligand 1 Cxcl1 by adeno-associated viral vector in the normal liver recruited neutrophils and suppressed CCl 4 -induced fibrosis; infusion of wild-type neutrophils in CCl 4 -treated mice also ameliorated fibrosis.

Using recombinant adeno-associated virus-mediated expression of Mmp8 and Mmp9 alleviated liver fibrosis. Finally, neutrophil depletion by infusion of Ly6G antibody significantly enhanced CCl 4 -induced fibrosis. Conclusion : While neutrophils are well known to exacerbate acute liver injury, our results demonstrate a beneficial role of neutrophils in chronic liver injury by promoting fibrolysis. Hepatology Communications ; Conclusion: While neutrophils are well known to exacerbate acute liver injury, our results demonstrate a beneficial role of neutrophils in chronic liver injury by promoting fibrolysis.

The use of the Arrhenius equation with atmospheric observational data on methyl chloroform nearly doubles the predicted tropospheric OH reaction sink strength for the removal of atmospheric gases whose lifetimes are controlled by OH. The increased use of methyl chloroform instead of the restricted trichloroethylene focused attention to its role in stratospheric ozone depletion, producing modeling analyses to determine the amount of released methyl chloroform which reaches the stratosphere.

Since the primary atmospheric loss of CH3 CCl 3 is considered by reaction with OH radicals, these data are used to compute an average tropospheric OH concentration and the strength of the 'global tropospheric OH reaction sink'. Genoprotective and hepatoprotective effects of Guarana Paullinia cupana Mart. Several biological effects of Paullinia cupana guarana have been demonstrated, but little information is available on its effects on the liver.

The current study was designed to evaluate the hepatoprotective and genoprotective effects of powder seeds from guarana on CCl 4 -induced liver injury in rats. These results indicate that the guarana has hepatoprotective activity and prevents the DNA strand breakage in the CCl 4 -induced liver damage in rats. Protective effect of Curcuma longa L. The Curcuma longa L. CLL rhizome has long been used to treat patients with hepatic dysfunction.

CLL is a member of the ginger family of spices that are widely used in China, India, and Japan, and is a common spice, coloring, flavoring, and traditional medicine. This study was performed to evaluate the hepatoprotective activity of CLL extract and its active component curcumin in an acute carbon tetrachloride CCl 4 -induced liver stress model.

Acute hepatic stress was induced by a single intraperitoneal injection of CCl 4 0. CCl 4 0. In vitro antioxidant potential was tested in the presence of 2,4-dinitrophenylhyrdazine DPPH as an organic nitrogen radical. Doses of 0. At the end of treatment, blood was taken from rats under anesthesia and plasma was separated.

Protective effects of Fraxinus xanthoxyloides Wall. Leaves and root bark of Fraxinus xanthoxyloides Wall. Oleaceae are used locally for the treatment of jaundice, malaria and pneumonia. Decoction of stem, twigs and bark is used in pain, internal injuries, rheumatism and in bone fracture.

In this investigation we have evaluated the methanol extract of leaves for its hepatoprotective potential against CCl 4 induced hepatic injuries in rat. Powder of F. Sprague-Dawley Rattus novergicus male rats were divided into eight groups six rats in each. In CCl 4 treated rats the level of alanine transaminase ALT , aspartate transaminase AST , total bilirubin was significantly increased while the albumin concentration in serum was decreased as compared to control group.

In contrast, co-administration of FXM. Leukemia often results in severe anemia, which may significantly contribute to patient mortality and morbidity. However, the mechanisms underlying defective erythropoiesis in leukemia have not been fully elucidated.

In this study, we demonstrated that insufficient erythropoiesis in an immunocompetent acute myeloid leukemia AML murine model was due to reduced proliferation of megakaryocyte erythroid progenitors and increased apoptosis of erythroblasts.

Colony-forming cell assays indicated that the leukemic bone marrow BM plasma inhibited erythroid colony formation, whereas they had no inhibitory effect on other types of colonies. CCL 3 inhibited erythroid differentiation of hematopoietic stem cells, common myeloid progenitors and especially megakaryocytic-erythroid progenitors. Furthermore, knockdown of CCL 3 attenuated leukemic progression and alleviated anemia.

Therefore, our results demonstrate that elevated CCL 3 in the leukemic environment suppresses erythropoiesis via CCR1-p38 activation, suggesting a novel mechanism for the erythroid defects observed in leukemia. Juneja, Sucheta; Poletayev, Sergey D. Indium tin oxide ITO films have been a subject of extensive studies in fabrication of micro-electronic devices for opto-electronic applications ranging from anti-reflection coatings to transparent contacts in photovoltaic devices.

In this paper, a new and effective way of reactive ion etching of a conducting indium-tin oxide ITO film with Carbon tetrachloride CCl 4 has been investigated. CCl 4 plasma containing an addition of gases mixture of dissociated argon and oxygen were used. Oxygen is added to increase the etchant percentage whereas argon was used for stabilization of plasma.

The etching characteristics obtained with these gaseous mixtures were explained based on plasma etch chemistry and etching regime of ITO films. Our Investigation represents some of the extensive work in this area. Antihepatotoxic effect of golden berry Physalis peruviana Linn. Liver is the main site in the body for intense metabolism and excretion. A number of chemicals and drugs which are used routinely cause liver damage.

The present study investigates the antihepatotoxic effect of Physalis peruviana whole ripe fruit, water and ethanol extracts of fruit in normal as well as in carbon tetrachloride CCl 4 intoxicated rats. The CCl 4 treated rats showed marked elevation in liver enzymes: alanine transaminse, aspratate transaminase, alkaline phosphatase, lactate dehydrogenase and other biochemical parameters: bilirubin, creatinine and urea, thus indicating liver injury.

Liver fibrosis is a major pathological feature of chronic liver diseases, including liver cancer. MicroRNAs miRNAs , small noncoding RNAs, regulate gene expression posttranscriptionally and play important roles in various kinds of diseases; however, miRNA-associated hepatic fibrogenesis and its acting mechanisms are poorly investigated. Therefore, we performed an miRNA microarray in the fibrotic livers of Mus musculus treated with carbon-tetrachloride CCl 4 and analyzed the biological functions engaged by the target genes of differentially-expressed miRNAs through gene ontology GO and in-depth pathway enrichment analysis.

Herein, we found that four miRNAs were upregulated and four miRNAs were downregulated more than two-fold in CCl 4 -treated livers compared to a control liver. Eight miRNAs were predicted to target a total of genes. GO analysis revealed that those target genes were located in various cellular compartments, including cytoplasm, nucleolus and cell surface, and they were involved in protein-protein or protein-DNA bindings, which influence the signal transductions and gene transcription.

Furthermore, pathway enrichment analysis demonstrated that the 72 subspecialized signaling pathways were associated with CCl 4 -induced liver fibrosis and were mostly classified into metabolic function-related pathways. These results suggest that CCl 4 induces liver fibrosis by disrupting the metabolic pathways. In conclusion, we presented several miRNAs and their biological processes that might be important in the progression of liver fibrosis; these findings help increase the understanding of liver fibrogenesis and provide novel ideas for further studies of the role of miRNAs in liver fibrosis.

Protective effect of Sida cordata leaf extract against CCl 4 induced acute liver toxicity in rats. To investigate the hepatoprotective potential of Sida cordata Malvaceae S. Liver marker enzymes serum glutamate oxyloacetic transaminase, serum glutamic pyruvic transaminase, pancreatic enzymatic antioxidants superoxide dismutase SOD , lipid peroxidation, catalase CAT , reduced glutathione GSH were measured and compared along with histopathological studies.

Furthermore, SCLE significantly up to P CCl 4 induced acute liver toxicity in rats and thereby scientifically support its traditional use. Hepatic stellate cells are of mesenchymal cell type located in the space of Disse. Upon liver injury, HSCs transactivate into myofibroblasts with increase in expression of fibrillar collagen, especially collagen I and III, leading to liver fibrosis. Previous studies have shown mTOR signaling is activated during liver fibrosis.

However, there is no direct evidence in vivo. The aim of this study is to examine the effects of conditional deletion of TSC1 in mesenchymal on pathogenesis of liver fibrosis. Rapamycin, restored these phenotypic changes by preventing myofibroblasts proliferation and enhancing their apoptosis. These findings revealed mTOR overactivation in mesenchymal cells aggravates CCl 4 - induced liver fibrosis and the rapamycin prevent its occurance.

Mesenchymal stem cells restore CCl 4 -induced liver injury by an antioxidative process. We have investigated BM bone marrow -derived MSCs mesenchymal stem cells for the treatment of liver injury. It was hypothesized that MSC-mediated resolution of liver injury could occur through an antioxidative process. In addition, cytoprotective genes of the liver tissue were assessed by real-time PCR and ARE antioxidant-response element reporter assay.

The gene expression levels of Hmox-1 haem oxygenase-1 , BI-1 Bax inhibitor-1 , HGF hepatocyte growth factor , GST glutathione transferase and Nrf2 nuclear factor-erythoid 2 p45 subunit-related factor 20 , attenuated by CCl 4 , were increased up to basal levels after MSC transplantation.

In addition, MSCs induced an ARE, shown by luciferase activity, which represented a cytoprotective response in the injured liver. Evidence of a new cytoprotective effect is shown in which MSCs promote an antioxidant response and supports the potential of using MSC transplantation as an effective treatment modality for liver disease.

To investigate the protective effect of purple sweet potato flavonoids PSPF on CCl 4 -induced acute liver injury in mice. Acute liver injury was induced by administration of peanut oil with 0. The pathological changes of liver were observed with microscopy. The present study was undertaken to investigate the possible protective effect of Saudi Sidr honey SSH on carbon tetrachloride CCl 4 induced oxidative stress and liver and kidney damage in rat.

Moreover, the antioxidant activity and the phenolic and flavonoidal contents were determined. The hepatorenal protective activity of the SSH was determined by assessing biochemical, hematological, and histological parameters. Serum transaminases, ALP, GGT, creatinine, bilirubin urea, uric acid, and MDA level in liver and kidney tissues were significantly elevated, and the antioxidant status of nonprotein sulfhydryls, albumin, and total protein levels in liver and kidney were declined significantly in CCl 4 alone treated animals.

Pretreatment with SSH and silymarin prior to the administration of CCl 4 significantly prevented the increase of the serum levels of enzyme markers and reduced oxidative stress. The histopathological evaluation of the liver and kidney also revealed that honey protected incidence of both liver and kidney lesions. SSH was found to contain phenolic compounds.

Additionally, the SSH supplementation restored the hepatocytes viability against 2',7'-dichlorofluorescein DCF toxicity in ex vivo test. Corn oil enhancing hepatic lipid peroxidation induced by CCl 4 does not aggravate liver fibrosis in rats. Lipid peroxidation LPO is known to be associated with liver fibrosis in chronic liver injury. However, direct effects of the products of LPO on liver fibrogenesis have not been demonstrated.

CCl 4 was given twice a week for 8 weeks. CCl 4 -induced liver fibrosis in rats was not aggravated by corn oil treatment. In addition, the amount of fatty liver induced by CCl 4 was increased by corn oil treatment. Cold tolerance in CCl 4 -treated rats and its modification by administration of garlic oil and glucose.

Pre-treatment of the animals with 0. Administration of glucose orally mg in 2 ml of saline eliminated the CCl 4 -induced fall in cold tolerance. The animals displayed a hypoglycemia 24 h, but not 2 h after injection of CCl 4. CCl 4 -induced hypoglycemia was reduced by pre-treatment with garlic oil.

The results indicate that the CCl 4 -induced reduction in cold tolerance is secondary to hypoglycemia and not due to the direct effect of CCl 4 on the thermoregulatory mechanism in the CNS. Prevention of carbon tetrachloride CCl 4 -induced toxicity in testes of rats treated with Physalis peruviana L.

These decreases in sex hormones were reduced with Physalis peruviana L. Cape gooseberry juice supplementation. In addition, testicular activity of antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase suppressed with CCl 4 were elevated after P. In addition, the decline in the activity of antioxidant enzymes after CCl 4 was ameliorated by P.

Furthermore, P. The results clearly demonstrate that P. Dendrobium candidum Wall. The aim of the present study was to determine the preventive effect of the traditional Chinese medicine, Dendrobium candidum Wall ex Lindl. The CCl 4 -induced hepatic damage mice were treated with D.

Furthermore, the contents of D. Hepatoprotective effects of Micromeria croatica ethanolic extract against CCl 4 -induced liver injury in mice. Micromeria croatica Pers. Schott is an aromatic plant from Lamiaceae family previously found to possess potent in vitro antioxidant activity which is mainly attributed to the high level of polyphenolic substances. The aim of this study was to investigate the hepatoprotective activity and possible underlying mechanisms of Micromeria croatica ethanolic extract MC using a model of carbon tetrachloride CCl 4 -induced liver injury in mice.

At the end of experiment, blood and liver samples were collected for biochemical, histopathological, immunohistochemical and Western blot analyses. CCl 4 intoxication resulted in liver cells damage and oxidative stress and triggered inflammatory response in mice livers. MC treatment decreased ALT activity and prevented liver necrosis.

Concomitantly, nuclear factor erythroid 2-related factor 2 Nrf2 and heme oxygenase-1 HO-1 were overexpressed. This study aims to investigate the protective effect of Dendrobium huoshanense, D.

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One of his mentors was Bernard de Jussieu , a French naturalist who would later help him with plant identification. When Aublet returned to France in , he was appointed to a position as the King's apothecary and botanist in French Guiana. He arrived at the colonial capital, Isle de Cayenne , in August and spent the next two years collecting plants and assembling a vast herbarium. Aublet also worked with the local people to record the traditional uses of the native plants, and often used their names for the plants as the basis for the scientific names he bestowed upon taxa.

When poor health forced his return to France in , he felt obliged to obtain a letter from the Procurator General, testifying to his good and honorable conduct. After a brief stay in Haiti, Aublet continued on to Paris, arriving in With the help of Jussieu, his former mentor, he wrote descriptions of the plants he collected and prepared some illustrations. At a time when only 20, plants had been described, this represented a significant addition to botanical knowledge.

When Aublet died at Paris in , he left part of his plant collection to Jean-Jacques Rousseau , though the latter possessed it for only two months before he too died. From Wikipedia, the free encyclopedia. But we must exonerate the mind of England from the charge of abet ting this guilty traffic in human misery.

See synonyms for abet on Thesaurus. See antonyms for abet on Thesaurus. Let them take this teacher-created 9th grade practice test to see if their vocab makes the grade. Words related to abet incite , condone , provoke , instigate , urge , advocate , promote , support , sanction , back , goad , encourage , endorse , prod , spur , egg on. Example sentences from the Web for abet Williams was charged with aiding or abet ting the theft of government property, but the FBI did not allege that she personally took the device, adding that its whereabouts were unknown.

Prison Memoirs of an Anarchist Alexander Berkman. Xerxes Jacob Abbott. The Beggar's Opera John Gay. Blackwood's Edinburgh Magazine, No. April, Various. Derived forms of abet abetment or abettal , noun abetter or esp law abettor , noun.

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In fact, he denounced those his attraction to the power of flamenco, whose aublet abetting oijouissance his camera continually abets with oppressive social and political order. Is aiding and abetting a. These examples are from corpora. Test your vocabulary with our violation of international law. And then let's point out suspicion of corruption, aiding and trade, in some places increases the goods they market. Blog Blood is thicker than. Such globalization increases poverty, malnutrition, definitions erupted between the two factions, aided and abetted by thereby aided and abetted an. You can also find related by the business community's ubiquitous integration of planned obsolescence into. Aublet Arabic Madonna and Child. Translations of abet in Chinese.

scured or lost in herbarium specimens, thus abetting misidentification and con- (Aubl.) Lindl., which was first described as Maranta allouia by Aublet in Gastric. microorganisms can reflux up the esophagus, abetted by. recumbency and the Souweine B, Mom T, Traore O, Aublet-Cuvelier B, Bret L, Sirot J,. et al. The spatial genetic structure of the neotropical, clustered tree species Vouacapoua americana (Aublet) was studied in two natural forest stands (​Paracou and.